Structural determination of the Nod factors produced by Rhizobium gallicum bv. gallicum R602 |
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Authors: | Soria-Díaz M Eugenia Rodríguez-Carvajal Miguel A Tejero-Mateo Pilar Espartero José L Morón Belén Sousa Carolina Megías Manuel Thomas-Oates Jane Gil-Serrano Antonio M |
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Institution: | Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Spain. |
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Abstract: | Rhizobium gallicum is a fast-growing bacterium found in European, Australian and African soils; it was first isolated in France. It is a microsymbiont which is able to nodulate plants of the genus Phaseolus. Rhizobium gallicum bv. gallicum R602 produces four extracellular signal molecules consisting of a linear backbone of N-acetyl glucosamine, bearing on the nonreducing terminal residue an N-methyl group and different N-acyl substituents. The four acyloligosaccharides terminate with a sulfated N-acetylglucosaminitol. This unit may be also acetylated. These structures were determined using carbohydrate and methylation analysis, mass spectrometric analysis and one-dimensional- and two-dimensional-nuclear magnetic resonance experiments. This work establishes the common structure that a lipochito-oligosaccharide must have so that the Rhizobium that produces and excretes it is able to nodulate plants of Phaseolus vulgaris. The substituents common to all the molecules are an N-methyl group and a C(18:1) fatty acid on the nonreducing terminal residue. |
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Keywords: | Rhizobium gallicum lipochito-oligosaccharide (LCO) structure of Nod factor |
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