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| 以减毒鼠伤寒沙门菌为载体的人呼吸道合胞病毒F蛋白DNA 疫苗滴鼻免疫抗体分析 | |
| 引用本文： | 程明,何金生,付远辉,乔伟,焦月盈.以减毒鼠伤寒沙门菌为载体的人呼吸道合胞病毒F蛋白DNA 疫苗滴鼻免疫抗体分析[J].微生物学报,2011,51(7):965-971. |
| 作者姓名： | 程明 何金生 付远辉 乔伟 焦月盈 |
| 作者单位： | 1. 安徽医科大学免疫学教研室,合肥,230032;北京交通大学生命科学与生物工程研究院,北京,100044 2. 北京交通大学生命科学与生物工程研究院,北京,100044 3. 安徽医科大学免疫学教研室,合肥,230032 |
| 基金项目： | 北京市自然科学基金(7092053) |
| 摘 要： | 目的]以减毒鼠伤寒沙门菌(Salmonella typhimurium aroA strain SL7207,SL7207)为载体携带可表达呼吸道合胞病毒(Human respiratory syncytial vrius,RSV)密码子优化的融合蛋白(Fusion glycoprotein,F)的真核表达质粒,探讨不同黏膜免疫途径及密码子优化对免疫效果的影响.方法]通过对RSV野生型F基因(Fwt)进行密码子优化,获得密码子优化的F基因(Fsyn),并构建可表达Fsyn的真核表达质粒pcDNA3.1/Fsyn,转化SL7207得到SL7207/pcDNA3.1/Fsyn.分别经滴鼻和灌胃途径,免疫BALB/c小鼠,采用间接ELISA方法比较免疫效果.结果]与灌胃组相比,滴鼻组诱导小鼠产生了更高水平的血清IgG和黏膜SIgA,获得了更好的免疫效果(P＜0.05).与野生型相比,密码子优化的F蛋白具有更好的免疫原性(P＜0.05).结论]经滴鼻途径免疫和密码子优化能够提高以SL7207为载体的RSV DNA疫苗免疫效果. |
| 关 键 词： | 人呼吸道合胞病毒 F蛋白密码子优化 黏膜免疫 减毒鼠伤寒沙门氏菌SL7207 |
| 收稿时间： | 2010/12/20 0:00:00 |
| 修稿时间： | 4/4/2011 12:00:00 AM |
Antibody responses induced by mucosal DNA vaccine encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium |
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| Ming Cheng,Jinsheng He,Yuanhui Fu,Wei Qiao and Yueying Jiao.Antibody responses induced by mucosal DNA vaccine encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium[J].Acta Microbiologica Sinica,2011,51(7):965-971. | |
| Authors: | Ming Cheng Jinsheng He Yuanhui Fu Wei Qiao and Yueying Jiao |
| Institution: | Ming Cheng1,2,Jinsheng He2,Yuanhui Fu2,Wei Qiao1,Yueying Jiao2 1Department of Immunology,Anhui Medical University,Hefei 230032,China 2College of Life Sciences & Bioengineering,Beijing Jiaotong University,Beijing 100044,China |
| Abstract: | Objective] In order to investigate antibody responses by musosal DNA vaccines encoding the codon-optimized F protein of human respiratory syncytial virus(RSV) delivered with attenuated Salmonella typhimurium aroA strain SL7207(SL7207) via intranasal or intragastric routes.Methods] After the codon-optimized F gene was synthesized,we constructed eukaryotic expression plasmid pcDNA3.1/Fsyn and transformed it into SL7207 to get recombinant SL7207 of SL7207/pcDNA3.1/Fsyn.Then,SL7207/pcDNA3.1/Fsyn was exploited... |
| Keywords: | human respiratory syncytial virus codon-optimized F gene mucosal administration attenuated Salmonella typhimurium aroA strain SL7207 |
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