Immunohistochemical analysis of EGF in epiphyseal growth plate from normal,hypophysectomized, and growth hormone-treated hypophysectomized rats |
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Authors: | Yoshifumi Tajima Kohtaro Kato Masanori Kashimata Masahiko Hiramatsu Nobuo Utsumi |
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Institution: | (1) Department of Oral Pathology, Meikai University School of Dentistry, 350-02 Sakado, Saitama, Japan;(2) Clinical Laboratory, Meikai University Hospital, 350-02 Sakado, Saitama, Japan;(3) Department of Dental Pharmacology, Meikai University School of Dentistry, 350-02 Sakado, Saitama, Japan |
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Abstract: | Epiphyseal growth plate cartilages from the proximal tibia of normal, hypophysectomized, and growth hormone (GH)-treated hypophysectomized rats were subjected to immunohistochemistry for detection of epidermal growth factor (EGF). In the normal growth plate, EGF was distributed mainly in the proliferative zone. Hypophysectomy resulted in considerable atrophy of the chondrocytes and the cartilage matrix (a decreased number of mature-type chondrocytes and a decreased ratio of proliferating to hypertrophic chondrocytes) and a significant diminution of EGF immunoreactivity. Treatment with GH reversed these effects of hypophysectomy, causing an increased thickness of the growth plate and EGF-reactive sites in all chondrocyte layers. The most intense immunostaining for EGF, however, was frequently seen in the nuclei of chondrocytes with flattened appearance. It appears that EGF could be incorporated or synthesized in chondrocytes having marked mitogenic activity. The present results, taken with previous data on EGF involvement in growth of cartilaginous tissue in vivo and in vitro, strongly suggest that EGF-immunoreactive chondrocytes are involved in cartilage proliferation and growth under the specific influence of GH. |
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Keywords: | EGF Cartilage Growth plate Hypophysectomy Growth hormone Immunohistochemistry Rat (Wistar) |
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