酒酒球菌苹果酸-乳酸酶基因的测序及分析

苹果酸乳酸酶是乳酸菌进行苹果酸乳酸发酵(MLF)的关键酶。以携带酒酒球菌(Oenococcusoeni)优良菌系OenococcusoeniSD2a的苹果酸乳酸酶基因mleA的重组质粒pLmleA作为测序质粒,进行测序分析。测序结果表明,克隆到的mleA基因序列与已报道的序列同源性为99%。mleA基因序列中有2个碱基与报道不同,其中1614碱基的改变导致错意突变,编码的氨基酸由报道的Asp变为Glu,这一改变使得原有的BamHI位点不再存在。

Sequencing and Analysis of Malo-Lactic Enzyme Gene from Oenococcus oeni

Malo lactic enzyme, code by mleA gene, is the key enzyme for malolactic fermentation (MLF). In this study a constructed plasmid pLmle A (Yep352:: mleA ) containing mleA from Oenococcus oeni SD 2a (a fine O.oeni strain from China) was used for sequencing. Sequence analysis showed that they had 99% similarity with the reported one. Two bases in the sequence of mleA shows difference from GenBank one. The amino acid coded in the position of 1614 is Glu instead of Asp, and the site of Bam HI does not exist for the base changing.;