The Nature of the Nucleotide at the 5' Side of the tRNA Su9 Anticodon Affects UGA Suppression in Escherichia coli |
| |
Authors: | Goldman-Levi Ronit; Kopelowitz June; Engelberg-Kulka Hanna |
| |
Institution: | Department of Molecular Biology, Hebrew University-Hadassah Medical School Jerusalem 91010, Israel |
| |
Abstract: | In Escherichia coli a UGA codon can be efficiently suppressedby a suppressor tRNATrp called Su9. Here, we show that the levelof UGA suppression is determined by the nature of the nucleotideat the 5' side of the anticodon of the suppressor (position33). UGA suppression occurs when a pyrimidine residue is locatedin position 33 of the tRNA, and suppression is more efficientwith a U than with a C in this position. On the other hand,when a purine residue is located at this position UGA suppressionis extremely low. These results show that in the case of tRNASu9, the UGA codon context effect does not require base pairingbetween the nucleotide at the 3' side of the codon and the 5'side of the anticodon. |
| |
Keywords: | genetic code nonsense suppression tRNA Escherichia coli |
本文献已被 Oxford 等数据库收录! |