Recognition of Carbohydrate Epitopes Specific for Electron-Dense Granule Antigens from Entamoeba histolytica by Monoclonal Antibodies in the Cecal Content of Infected Hamsters |
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Authors: | Sergio Arias-Negrete Gloria León Fernando Anaya-Velázquez Rosalinda Tovar Miguel Angel Moreno José Manuel Hernández María de Lourdes Muñoz |
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Institution: | (1) University of Cincinnati College of Medicine, Department of Pathology & Laboratory Medicine, P.O. Box 670529,, Cincinnati, OH 45267-0529, USA, US |
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Abstract: | Saccharomyces cerevisiae CGR1 encodes a 120-amino acid protein with a predominant nucleolar localization. In this study we report the identification and
cloning of the ortholog, cgrA, from Aspergillus nidulans. The cgrA gene is comprised of three exons on A. nidulans Chromosome 7. The cDNA contains a single open reading frame (ORF) that would encode a protein of 114 amino acids with 44%
sequence identity to yeast Cgr1p. A plasmid expressing cgrA complemented the impaired growth phenotype of a yeast strain that can be inducibly depleted of CGR1, and a green fluorescent protein (GFP)-tagged CgrA protein had the same nucleolar localization as the corresponding yeast
protein. These results identify cgrA as the A. nidulans ortholog of yeast CGR1 and suggest evolutionary conservation of nucleolar localization mechanisms used by these proteins.
Received: 14 September 2000 / Accepted: 13 November 2000 |
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