Recovery of uptake and assimilation of nitrate in Scenedesmus sp. previously exposed to elevated levels of Cu2+ and Zn2+ |
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Authors: | Tripathi Bhumi Nath Mehta Surya Kant Gaur Jai Prakash |
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Institution: | Laboratory of Algal Biology, Department of Botany, Banaras Hindu University, Varanasi 221 005, India. |
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Abstract: | A study of the effects of elevated levels of Cu2+ and Zn2+ on NO3- uptake and nitrate reductase (NR) activity in Scenedesmus sp. was carried out. The two metals inhibited NR and NO3- uptake in a concentration-dependent manner, with the latter process being inhibited more strongly than the former. After withdrawal of metal stress, NR activity and NO3- uptake recovered in a metal ion concentration-dependent manner. Dark pretreatment of the alga enhanced the toxic effects of the metal ions on NR activity and NO3- uptake. The recovery from metal stress was slower in the dark-pretreated cells in comparison to the light-pretreated cells. No recovery of NR and NO3- uptake occurred in the presence of the photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), suggesting that photosynthesis was required for the recovery from metal stress. Cycloheximide blocked the recovery of NR activity in metal-treated alga, suggesting that new enzyme synthesis was required for the recovery from metal stress. |
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