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Indoleacetic acid enhancement of 14C methionine uptake by tomato cell cultures aids assay of pathogen extracts
Authors:Clarence Madhosingh  William R. Jarvis
Affiliation:(1) Lordon Research Centre, Agriculture Canada, 1400 Western Road, N6G 2V4 London, Ontario, Canada;(2) Research Station, Agriculture Canada, NOR 1GO Harrow, Ontario, Canada
Abstract:Incorporation of one micromolar IAA in the assay system increased the rate of 14C methionine uptake by tomato cells in suspension and effectively differentiated the rates of uptake such that cells treated with pathogenic and non-pathogenic Fusarium extracts could be easily distinguished in a rapid 3 h assay.
Keywords:cell suspension cultures  Fusarium wilt  rapid disease assay  tomato
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