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Structural studies of the Rhizobium trifolii extracellular polysaccharide
Authors:Per-Erik Jansson  Bengt Lindberg  Hans Ljunggren
Affiliation:Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, S-106 91 Stockholm Sweden;Department of Microbiology, Swedish University of Agricultural Sciences, S-75O 07 Uppsala Sweden
Abstract:The structure of the extracellular polysaccharide of Rhizobium trifolii has been investigated. Methylation analysis, sequential degradations by oxidation and elimination of oxidized residues, uronic acid degradation, and degradation by oxidation of the acetylated polysaccharide with chromium trioxide in acetic acid were the main methods used. It is proposed that the polysaccharide is composed of heptasaccharide repeating-units having the following structure:
/></figure> An unusual feature is that some of the repeating units are incomplete and lack the terminal β-d-galactopyranosyl group. The polysaccharide contains <em>O</em>-acetyl groups (somewhat more than 1 mol. per unit), linked to O-2 and O-3 of 4-<em>O</em>-substituted d-glucopyranosyl chain-residues. A previous finding that the polysaccharide contains 2-deoxy-d-<em>arabino</em>-hexose (2-deoxy-d-glucose) residues is erroneous.</td>
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