Spin-labelling of sialic acid in soluble and cell surface glycoproteins |
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| Authors: | John D Aplin DE Brooks Charles FA Culling Laurance D Hall Philip E Reid |
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| Institution: | Department of Chemistry and Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, B.C., V6T 1W5 Canada |
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| Abstract: | A new, mild method is described for spin-labelling sialic acid residues in situ. The procedure involves the formation of C-1 sialamides and has been applied to a serum glycoprotein, a mucin, tissue sections from human colon, and erythrocyte membrane components. The selectivity of the method and its possible applicability to other types of labelling are discussed. |
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