DIGE compatible labelling of surface proteins on vital cells in vitro and in vivo |
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| Authors: | Mayrhofer Corina Krieger Sigurd Allmaier Günter Kerjaschki Dontscho |
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| Institution: | Department of Clinical Pathology, Medical University of Vienna, Vienna, Austria. |
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| Abstract: | Efficient methods for profiling of the cell surface proteome are desirable to get a deeper insight in basic biological processes, to localise proteins and to uncover proteins differentially expressed in diseases. Here we present a strategy to target cell surface exposed proteins via fluorescence labelling using CyDye DIGE fluors. This method has been applied to human cell lines in vitro as well as to a complex biological system in vivo. It allows detection of fluorophore-tagged cell surface proteins and visualisation of the accessible proteome within a single 2-D gel, simplifying subsequent UV MALDI-MS analysis. |
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