| hEPO cDNA重组逆转录病毒的构建 |
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| 引用本文： | 瞿成奎,魏汉东,贺福初,王立生,吴祖泽.hEPO cDNA重组逆转录病毒的构建[J].生物化学与生物物理进展,1996,23(2):150-153. |
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| 作者姓名： | 瞿成奎 魏汉东 贺福初 王立生 吴祖泽 |
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| 作者单位： | 军事医学科学院放射医学研究所;军事医学科学院放射医学研究所;军事医学科学院放射医学研究所;军事医学科学院放射医学研究所;军事医学科学院放射医学研究所 |
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| 基金项目： | 国家自然科学基金资助课题. |
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| 摘 要： | 通过DNA重组技术，将不含非编码区的hEPO cDNA片段重组到逆转录病毒质粒pLXSN, pLNCX中重组质粒转染PA317细胞后，经G418筛选，抗性克隆细胞培养上清能成功地感染NIH3T3细胞，使之在筛选培养基中形成典型的G418抗性克隆，该克隆细胞染色体中成功地整合了EPOcDNA，并且表达出有生物学活性的红细胞生成素(EPO)产物。
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| 关 键 词： | 红细胞生成素 重组逆转录病毒载体 基因转移 |
| 收稿时间： | 1995/3/15 0:00:00 |
| 修稿时间： | 1995/9/22 0:00:00 |
Construction of hEPO cDNA Recombinant Retrovirus |
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| Qu Chengkui,Wei Handong,He Fuchu,Wang Lisheng and Wu Zuze.Construction of hEPO cDNA Recombinant Retrovirus[J].Progress In Biochemistry and Biophysics,1996,23(2):150-153. |
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| Authors: | Qu Chengkui Wei Handong He Fuchu Wang Lisheng and Wu Zuze |
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| Institution: | BeiJing Institute of Radiation Medicine;BeiJing Institute of Radiation Medicine;BeiJing Institute of Radiation Medicine;BeiJing Institute of Radiation Medicine;BeiJing Institute of Radiation Medicine |
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| Abstract: | hEPO cDNA without non-coding ration was recombined with retrovirus vector pLXSN, pLNCX through DNA recombinant techniques. After these two recombinant plasmids were transfeeted the retrovirus packaging cell line PA317, G418 resistant clones could produce defective EPO cDNA recombinant retrovirus successfully which could infect NIH 3T3 target cells and make them form typical resistant clones in G418 selective medium. Moreover, in the genome of these infected target cells, hEPO cDNA was successfully integrated and expressed. |
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| Keywords: | hEPO recombinant retrovirus gene transfer |
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