An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes |
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| Authors: | F Bruyant M Babin A Sciandra D Marie B Genty H Claustre J Blanchot A Bricaud R Rippka S Boulben F Louis F Partensky |
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| Institution: | (1) Laboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS, ESA 7077, BP 8, 06238 Villefranche-sur-Mer CEDEX, France;(2) Laboratoire d'Océanographie Biologique et Ecologie du Plancton Marin, Université Pierre et Marie Curie and CNRS, BP 28, 06234 Villefranche-sur-Mer CEDEX, France;(3) Station Biologique, Université Pierre et Marie Curie and CNRS, BP 74, 29680 Roscoff, France;(4) Laboratoire d'Ecophysiologie Végétale, Université Paris Sud and CNRS, UPRESA 8079, 91405 Orsay, France;(5) Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Unité de Physiol Microbienne (CNRS URA 1129), 28 Rue du Dr. Roux, 75724 Paris CEDEX 15, France |
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| Abstract: | A cyclostat was designed for growing the oceanic oxyphotobacterium Prochlorococcus PCC 9511. Culture of this organism, known to bedifficult to grow, was mastered for a large volume. Prochlorococcusgrew well and axenic conditions were maintained for up to 15 days. Wedesigned an illumination system allowing a smooth bell-shaped irradiancecurve reaching almost 1000  mol quanta m-2 s-1 tobe obtained. Cell division was strongly synchronised under theseillumination conditions, which were close to those found at low latitude inthe upper layer of ocean. The described device is particularly well suited tomake experiments requiring up to 6 L per day of well synchronised,exponentially-growing Prochlorococcus culture. |
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| Keywords: | cyclostat Prochlorococcus culture axenic cycle phytoplankton |
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