The thiobarbituric acid-reactivity of bile pigments |
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| Authors: | J M Gutteridge T R Tickner |
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| Affiliation: | 1. Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China;2. Department of Translational Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA;3. Department of Leukemia, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA;1. Department of Chemistry, Tunghai University, Taichung 40704, Taiwan;2. Life Science Research Center, Tunghai University, Taichung 40704, Taiwan |
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| Abstract: | Biliverdin reacts with thiobarbituric acid to give a red chromogen with absorption peaks at 560, 530 and 465 nm in acid solution and 602, 566 and 482 nm in alkaline solution. The reaction in acid solution could interfere with lipid peroxidation studies in which malondialdehyde is measured at 532 nm. The TBA reaction in biological fluids is discussed together with its use as a possible measure of bile pigments. |
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