GM-CSF accelerates proliferation of endothelial progenitor cells from murine bone marrow mononuclear cells in vitro |
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Authors: | Qi Ru Wang Feng Wang Wen Biao Zhu Jun Lei Yan Hong Huang Bao He Wang Qi Yan |
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Institution: | aDepartment of Physiology, Xiang Ya Medical College, Central South University, Changsha 410078, China;bCollege of Life Science, Hunan Normal University, Changsha 410081, China;cEditorial Department of Journal of Clinical Research, Changsha 410008, China;dBenaroya Research Institute, Departments of Biological Structure and Ophthalmology, University of Washington, Seattle, WA 98195, USA |
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Abstract: | Objective: To test whether the GM-CSF accelerates the proliferation of bone marrow endothelial progenitor cells (BM EPCs). Methods: BM EPCs were induced by endothelial cell conditioned medium (EC-CM). The effect of different concentrations of GM-CSF on the proliferation of BM EPCs was evaluated by the formation of EC-cols, MTT assay, and cell cycle assay. The single progenitor cell growth curves were quantified. Results: The data indicated that GM-CSF accelerated the proliferation of BM EPCs both in colony numbers and colony size. MTT confirmed the effect of GM-CSF on accelerating the proliferation of BM EPCs. The single colony experiments showed that EC-cols expressed different proliferation capacity, suggesting that the EC-cols with different proliferation potentials might have been derived from different levels of immature progenitors. The cell cycle assay showed that the rate of cells entering into S phase was 9.3% in the group treated with GM-CSF and 2.1% in the controls. Furthermore, these cells displayed the specific endothelial cell markers and formed capillary-like structures. Conclusions: GM-CSF accelerates proliferation of BM EPCs. The potential beneficial of GM-CSF in the application of treating vascular ischemic patients is promising. |
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Keywords: | Granulocyte-macrophage colony stimulating factor Endothelial progenitor cells Bone marrow mononuclear cells Proliferation |
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