Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility |
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| Authors: | Rodnay Sormani Lei Yao Benoît Menand Najla Ennar Cécile Lecampion Christian Meyer Christophe Robaglia |
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| Affiliation: | 1.DSV-DEVM Laboratoire de Génétique et de Biophysique des Plantes,UMR 6191 CNRS-CEA-Université de la Méditerranée, Faculté des Sciences de Luminy,Marseille,France;2.Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing,China;3.Cell & Developmental Biology Department, John Innes Centre,Colney, Norwich,UK;4.Unité de Nutrition Azotée des Plantes,Institut Jean-Pierre Bourgin (IJPB) INRA,VERSAILLES Cedex,France |
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| Abstract: | Background The eukaryotic TOR pathway controls translation, growth and the cell cycle in response to environmental signals such as nutrients or growth-stimulating factors. The TOR protein kinase can be inactivated by the antibiotic rapamycin following the formation of a ternary complex between TOR, rapamycin and FKBP12 proteins. The TOR protein is also found in higher plants despite the fact that they are rapamycin insensitive. Previous findings using the yeast two hybrid system suggest that the FKBP12 plant homolog is unable to form a complex with rapamycin and TOR, while the FRB domain of plant TOR is still able to bind to heterologous FKBP12 in the presence of rapamycin. The resistance to rapamycin is therefore limiting the molecular dissection of the TOR pathway in higher plants. |
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