Quantification of the tissue-culture induced variation in barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) |
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Authors: | Piotr T Bednarek Renata Orłowska Robert MD Koebner Janusz Zimny |
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Institution: | 1.Plant Breeding & Acclimatization Institute,B?onie,Poland;2.UWM,Olsztyn,Poland;3.John Innes Centre,Colney Lane,UK |
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Abstract: | Background When plant tissue is passaged through in vitro culture, many regenerated plants appear to be no longer clonal copies of their donor genotype. Among the factors that affect
this so-called tissue culture induced variation are explant genotype, explant tissue origin, medium composition, and the length
of time in culture. Variation is understood to be generated via a combination of genetic and/or epigenetic changes. A lack
of any phenotypic variation between regenerants does not necessarily imply a concomitant lack of genetic (or epigenetic) change,
and it is therefore of interest to assay the outcomes of tissue culture at the genotypic level. |
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