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Somaclonal variation inLolium multiflorum L. andL. temulentum L.
Authors:J A Jackson  P J Dale
Institution:(1) Institute for Grassland and Animal Production, Welsh Plant Breeding Station, Plas Gogerddan, SY23 3EB Aberystwyth, Dyfed, United Kingdom;(2) Present address: Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, Saskatoon, Saskatchewan, Canada, S7N OW9, Canada;(3) Present address: Institute of Plant Science Research, Cambridge Laboratory, Maris Lane, Trumpington, CB2 2JB Cambridge, United Kingdom
Abstract:Tiller-derived callus ofLolium multiflorum L. gave rise to 65 regenerants displaying variation in height, flowering time, chromosome number and alteration to the banding pattern of the isoenzyme superoxide dismutase. Plants were also regenerated from callus cultures initiated from immature embryos of the related inbreeding speciesLolium temulentum L. Progeny of the regenerated plants from this species displayed variation in height, flowering date, ear length and chlorophyll content.Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid - IAA indole-3-acetic acid - BAP 6-benzylaminopurine
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