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| 利用S-SAP鉴定富士苹果芽变 | |
| 引用本文： | 贾怀志,蔡斌华,王飞,孙俊,王昆,章镇. 利用S-SAP鉴定富士苹果芽变[J]. 西北植物学报, 2009, 29(6) |
| 作者姓名： | 贾怀志 蔡斌华 王飞 孙俊 王昆 章镇 |
| 作者单位： | 1. 南京农业大学,园艺学院,南京,210095 2. 安徽农业大学,园艺学院,合肥,230036 3. 中国农业科学院,果树研究所,辽宁兴城,125100 |
| 摘 要： | 利用银染S-SAP技术对苹果'富士'及其14个芽变品种进行研究,结果发现,筛选出的6对引物中,L12/E7能够将所有'富士'芽变品种同'富士'区分开来,其余5对引物能够在芽变品种中区分'秋富5号'、'烟富1'、'盛放富1'和'长富1号'.由于S-SAP多态性主要由基因组内逆转座子的转座引起,因此'富士'苹果芽变品种的产生可能与逆转座子的插入有关. |
| 关 键 词： | 富士 芽变 鉴定 |
Identification of Fuji Mutant Sprouts by S-SAP |
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| JIA Huai-zhi,CAI Bin-hu,WANG Fei,SUN Jun,WANG Kun,ZHANG Zhen. Identification of Fuji Mutant Sprouts by S-SAP[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(6) | |
| Authors: | JIA Huai-zhi CAI Bin-hu WANG Fei SUN Jun WANG Kun ZHANG Zhen |
| Affiliation: | JIA Huai-zhi1,CAI Bin-hua1,WANG Fei1,SUN Jun2,WANG Kun3,ZHANG Zhen1 (1 College of Horticulture,Nanjing Agticultural University,Nanjing 210095,China,2 College of Horticulture,Anhui Agricultural University,Hefei 230036,3 Research Institute of Pomology,CAAS,Xingcheng,Liaoning 125100,China) |
| Abstract: | 'Fuji' apple and its 14 bud mutants were identified with Silver staining S-SAP (Sequence-specific amplification polymorphism).In six primer pairs screened from 44 primer combinations,L12/E7 could discriminate all the bud mutants from maternal 'Fuji' successfully,other 5 primer pairs could discriminate 'Akifu 5','Yanfu 1','Shengfangfu 1' and 'Nagafu 1' mutants.The polymorphism in S-SAP mainly comes from the transposition of retrotransposon.The results suggested that the bud mutants generated in 'Fuji' appear... |
| Keywords: | S-SAP |
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