Genomic DNA sequence of Rhesus (M. mulatta) cystic fibrosis (CFTR) gene |
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| Authors: | Jeffrey J Wine Damjan Glavač Gregory Hurlock Clare Robinson Margaret Lee Uros Potočnik Metka Ravnik-Glavač Michael Dean |
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| Institution: | (1) Cystic Fibrosis Research Laboratory, Bldg. 420 (Main Quad), Stanford University, Stanford, California 94305-2130, USA, US;(2) Laboratory of Molecular Pathology, University of Ljubljana Korytkova 2, Ljubljana, Slovenia, YU;(3) Human Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute–Frederick Cancer Research and Development Center, Bldg. 560, Rm. 21-18, Frederick, Maryland 21702, USA, US |
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| Abstract: | Cystic fibrosis is a common human genetic disease caused by mutations in CFTR, a gene that codes for a chloride channel that
is regulated by phosphorylation and cytosolic nucleotides. As part of a program to discover natural animal models for human
genetic diseases, we have determined the genomic sequence of CFTR in the Rhesus monkey, Macaca mulatta. The coding region of rhesus CFTR is 98.3% identical to human CFTR at the nucleotide level and 98.2% identical and 99.7% similar at the amino acid level. Partial
sequences of flanking introns (5582 base pair positions analyzed) revealed 91.1% identity with human introns. Relative to
rhesus intronic sequence, the human sequences had 27 insertions and 22 deletions. Primer sequences for amplification of rhesus
genomic CFTR sequences are provided. The accession number is AF013753 (all 27 exons and some flanking intronic sequence).
Received: 27 August 1992 / Accepted: 5 December 1997 |
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