Plant regeneration from callus cultures in two ecotypes of reed (Phragmites communis Trinius) |
| |
Authors: | Suxia Cui Wei Wang Chenglie Zhang |
| |
Institution: | (1) Institute of Botany & Plant Physiology, School of Life Science, Lanzhou University, 730000 Lanzhou, Gansu, P. R. China |
| |
Abstract: | Summary Different ecotypes of reed (Phragmites communis Trinius) provide an ideal resource for studies on plant environmental adaptations and presence of genes relating to stress
resistance. Dune reed is a drought-tolerant reed ecotype growing in the desert regions of north-west China. In this work,
in vitro culture systems of dune reed and local swamp reed (as control) were established by optimizing the culture conditions for
each of them. Bright yellow calluses were induced on a Murashige and Skoog medium containing 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 5.4 μM naphthaleneacetic acid and 2.2μM benzyladenine. Benzyladenine promoted callus induction, but was not required for callus maintenance. Four types of callus
have been identified from each of the reed ecotypes. Two types of callus, i.e. type A (formed normal green shoots) and type
C (formed albino plants), were both found as embryogenic calluses. The optimal concentrations of 2,4-D to maintain embryogenic
callus were 2.3–4.5 μM for dune reed and 9.0–13.5 μM for swap reed. Plant regeneration was achieved from types A and C callus in a hormone-free medium. The embryogenic calluses
of swamp reed have been maintained for over 2 yr and still retain their strong embryogenic potential; however, those of dune
reed gradually lost their embryogenic potential after only 7 mo. of culture. Regenerated plants from the two reed ecotypes
showed, after a growth season, similar morphology and the same chromosome number (2n=8x=96, octoploid) as the wild plants. |
| |
Keywords: | dune reed Gramineae in vitro culture somatic embryogenesis swamp reed |
本文献已被 SpringerLink 等数据库收录! |
|