Transformation of peas |
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Authors: | D R Davies J Hamilton P Mullineaux |
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Institution: | (1) John Innes Centre for Plant Science Research, Colney Lane, NR4 7UH Norwich, UK |
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Abstract: | Summary The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a -glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analysis and tests for -glucuronidase activity. The plants were fertile and tests of their progeny confirmed the transmission of integrated sequences through a sexual generation. This transformation method has the merit of an unlimited supply of material for inoculation and a relatively short time scale from inoculation to the production of rooted plants.Abbreviations BAP
benzyl amino purine
- GA3
gibberellic acid
- GUS
=glucuronidase
- IBA
indole butyric acid
- MS
Murashige and Skoog medium
- NAA
naphthalene acetic acid
- NPTII
neomycin phosphotransferase II
- PCR
polymerase chain reaction
- TO
first generation transformants
- T1
second generation transformants |
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Keywords: | Peas Seed Transformation Agrobacterium Meristems |
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