| 适用于水稻叶片蛋白质组分析的双向电泳技术 |
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| 引用本文： | 王玉琪,彭新湘.适用于水稻叶片蛋白质组分析的双向电泳技术[J].植物生理与分子生物学学报,2006,32(2):252-256. |
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| 作者姓名： | 王玉琪 彭新湘 |
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| 作者单位： | 华南农业大学生命科学学院分子植物生理研究室,广州,510642 |
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| 基金项目： | 中国科学院资助项目;华南农业大学校科研和教改项目 |
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| 摘 要： | 针对水稻叶片中含有大量色素和酚等干扰物质的现象，通过对水稻叶片蛋白提取方法、上样量和聚丙烯酰胺凝胶浓度等方面做了必要改进，建立了一套适用于水稻叶片蛋白质组分析的双向电泳（2-DE）方法。
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| 关 键 词： | 蛋白质组 双向电泳 水稻 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005-12-01 |
A Two-Dimensional Electrophoresis Protocol Suitable for Proteomic Study of Rice Leaves |
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| WANG Yu-Qi,PENG Xin-Xiang.A Two-Dimensional Electrophoresis Protocol Suitable for Proteomic Study of Rice Leaves[J].Journal Of Plant Physiology and Molecular Biology,2006,32(2):252-256. |
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| Authors: | WANG Yu-Qi PENG Xin-Xiang |
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| Institution: | Lab of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | A 2-DE (two-dimensional electrophoresisprotocol suited for the separation of proteins fromrice leaves was established. Protein extractionquantitative loading of samples and concentrationsof the gel were modified and improved to minimize markedly the interference by leaf pigmentsand other non-protein substances, thus resulting insatisfactory separation results. |
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| Keywords: | proteome two-dimensional electrophoresis rice |
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