Sesbania rostrata root and stem nodule leghemoglobins: purification, and relationships among the seven major components |
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| Authors: | D Bogusz A A Kortt C A Appleby |
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| Affiliation: | 1. Geo-Biodiversity and Natural Patrimony Laboratory (GEOBIO), Scientific Institute, “Geophysics, Natural Patrimony and Green Chemistry” Research Center (GEOPAC), Mohammed V University in Rabat, Avenue Ibn Batouta, P.B. 703, 10106 Rabat-Agdal, Morocco;2. UFR of Geological and Mining Sciences, University of Man., BP 20, Man, Côte d''Ivoire;3. Laboratory of Cartography and Digital Technology, Department of Earth Sciences, Faculty of Sciences, University Abdelmalek Essaâdi, Tétouan, Morocco;1. Laboratory of Bioinorganic Chemistry, Department of Organic and Inorganic Chemistry, Federal University of Ceara, Center for Sciences, Fortaleza, Ceara 60440-900, Brazil;2. Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA;1. School of Psychology, Flinders University, Adelaide, SA, Australia;2. Centre for Cognitive Neuroscience, Neuroscience and Behavioral Disorders Program, Duke-NUS Medical School, Singapore, Singapore |
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| Abstract: | By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found in photosynthetic nodules. All components of Sesbania Lb are monomeric, with molecular weights varying between 15,000 and 17,000, and at least six of them are separate gene products. It is suspected that variable conjugation with nonprotein moieties might be partially responsible for the molecular weight differences and anomalous behavior observed between isoelectric focusing and anion-exchange chromatography. |
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