The identification of 3-methylcholanthrene-9,10-dihydrodiol as an intermediate in the binding of 3-methylcholanthrene to DNA in cells in culture |
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Authors: | H. W. S. King M. R. Osborne P. Brookes |
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Affiliation: | Chemical Carcinogenesis Division, Institute of Cancer Research, Pollards Wood Research Station, Nightingales Lane, Chalfont St. Giles, Buckinghamshire HP8 4SP, United Kingdom |
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Abstract: | Two metabolites of 3-methylcholanthrene (3MC), previously shown to be precursors of the DNA-bound form of 3MC observed in embryo cells in culture, were prepared from 3MC by microsomal metabolism and isolated by high pressure liquid chromatography (HPLC). From HPLC analysis of the metabolites of 3MC, from mass spectrometric analysis and from comparison with the fluorescence spectra of all 5 possible dihydrodiols of the alkylated benzanthracenes, it was deduced that one of the precursor metabolites was a 9,10-dihydrodiol of 3MC while the other was a 1 or 2-hydroxy derivative thereof. |
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Keywords: | BA-3,4-diol benz(a)anthracene-3,4-dihydrodiol 7MBA 7-methylbenz(a)anthracene 3MC 3-methylcholanthrene HPLC high pressure chromatography |
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