Dynamic light scattering study of calcium-induced fusion in phospholipid vesicles |
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Authors: | EP Day JT Ho RK Kunze Jr ST Sun |
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Institution: | Department of Physics and Astronomy, State University of New York at Buffalo, Amherst, N.Y. 14260, U.S.A. |
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Abstract: | Acidic sonicated phospholipid vesicles can undergo dramatic morphological changes due to fusion in the presence of divalent metal ions. For example, small spherical phosphatidylserine vesicles can form scroll-like cylinders which precipitate in the presence of Ca2+ above a threshold concentration. Subsequent addition of EDTA will yield large, unilamellar vesicles. These events have previously been established through the combined use of differential scanning calorimetry and freeze-fracture electron microscopy. We have applied the technique of dynamic light scattering to follow these fusion events rapidly, accurately, and non-perturbatively as they occur in solution at calcium concentrations slightly below threshold for precipitation. |
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