基因工程技术构建预防性HPV疫苗

宫颈癌的发生与高危型人乳头瘤病毒（human papillomavirus，HPV）感染高度相关已得到公认。利用基因重组技术开发HPV疫苗是目前预防宫颈癌唯一有效的方法。文章综述了近年来在基因工程HPV疫苗研制中广泛应用的载体表达系统及其特点、依据密码子偏爱原则优化病毒外壳蛋白基因序列显著提高外壳蛋白产量等方面的研究成果，以为进一步研制与优化HPV疫苗提供参考。

Construction of prophylactic HPV vaccines using genetic engineering technology

It's commonly accepted that the infection of HPV with high-risk is the important factor in the occurrence of cervix cancer. Recombinate technologies are used to develop HPV vaccines, which is the only effective method to prevent cervix cancer so far. From the both aspects of expression vectors and codon optimization, this article reviewed the widely used expression vectors in recent years and their characteristics ；as well as the research results on obviously enhancement of capsid protein by optimizing the virus capsid gene according to codon preference, which provide basis for the further manufacturing and optimization of HPV vaccines.