| Abstract: | High-specific-activity D-3-3H]pantothenic acid (5 Ci/mmol) was prepared from commercially available beta-3-3H]alanine employing Escherichia coli strain DV1 (panD2 pan F1). This strain is defective in beta-alanine synthesis and pantothenate uptake, and under appropriate growth conditions converted 85 to 90% of the input beta-3-3H]alanine to extracellular D-3-3H]pantothenate. The radiolabeled vitamin was purified from the medium by thin-layer chromatography followed by reverse-phase high-performance liquid chromatography. The overall yield of D-3-3H]pantothenic acid was 30% and radiochemical purity was greater than 99%. |
