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Determination of beta-carboline alkaloids in fruiting bodies of Hygrophorus spp. by liquid chromatography/electrospray ionisation tandem mass spectrometry
Authors:Teichert Axel  Lübken Tilo  Schmidt Jürgen  Kuhnt Christine  Huth Manfred  Porzel Andrea  Wessjohann Ludger  Arnold Norbert
Affiliation:Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.
Abstract:The beta-carboline alkaloids harmane (1) and norharmane (2) were isolated from fruiting bodies of Hygrophorus eburneus (Bull.) Fr. as well as brunnein A (3) from Hygrophorus hyacinthinus Quél. (Tricholomataceae, Agaricales) for the first time. Their occurrence within the genus was investigated using liquid chromatography/electrospray ionisation tandem mass spectrometric methods, especially by selected reaction monitoring. Based on these results their chemotaxonomical relevance is discussed.
Keywords:LC/ESI‐SRM  harmane  norharmane  brunnein A  Hygrophorus  Basidiomycetes
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