| 筛选抗甲3型（H3N2）流感病毒单克隆抗体ELISA间接方法的建立 |
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| 引用本文： | 张颖超,席建华.筛选抗甲3型（H3N2）流感病毒单克隆抗体ELISA间接方法的建立[J].生物技术,1993,3(3):44-46. |
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| 作者姓名： | 张颖超 席建华 |
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| 作者单位： | 大连市卫生防疫站 大连
(张颖超，席建华)，大连市卫生防疫站 大连(王秀萍) |
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| 摘 要： | 用血凝抑制实验方法,虽可直接筛选到抗甲_3型流感病毒血凝素(H)单克隆抗体,但检测出的杂交瘤培养物上清中有小牛血清,做血凝抑制时还需用受体破坏酶处理去除非特异性抑制物。为减少麻烦我们建立了便于大批检测和筛选抗甲_3型(H_3N_2)流感病毒单克隆抗体的ELISA间接方法。
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| 关 键 词： | 杂交瘤 单克隆抗体 流感病毒 |
Screening Anti-Influenza Yirus H_3N_2 Monoclonal Antibody by Indirect ELISA Method |
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| Zhang Yingchao Xi Jianhua Wang Xiuping.Screening Anti-Influenza Yirus H_3N_2 Monoclonal Antibody by Indirect ELISA Method[J].Biotechnology,1993,3(3):44-46. |
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| Authors: | Zhang Yingchao Xi Jianhua Wang Xiuping |
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| Abstract: | Anti-hemagglutinin monoclonal antibody of Influenza virus H3N2 can be screened directly by hemagglutination inhibition technique. But there is bovine serum albumin in the hybridoma cell culture. So we need receptor-destroying enzyme to get out of nonspecific inhibitors. To eliminate this trouble and detect a good number of samples, we have established an indirect ELISA method for screening anddetecting anti-influenza virus H3N2 monoclonal antibody |
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| Keywords: | Hybridoma cell Monoclonal antibody |
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