Laser-assisted microinjection into targeted animal cells |
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| Authors: | Mohanty Samarendra K Sharma Mrinalini Gupta Pradeep K |
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| Institution: | (1) Biomedical Applications Section Center for Advanced Technology, Indore, 452013, India |
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| Abstract: | A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer. |
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| Keywords: | microinjection optoporation photodynamic treatment transfection |
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