Understanding mutations and protein stability through tripeptides |
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Authors: | Anishetty Sharmila Anishetty Ramesh Pennathur Gautam |
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Affiliation: | Centre for Biotechnology, Anna University, Chennai 600 025, India. |
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Abstract: | A novel methodology to predict the local conformational changes in a protein as a consequence of missense mutations is proposed. A pentapeptide at the locus of mutation plays the dominant role and it is analyzed in terms of tripeptides. A measure for spatial and temporal fluctuations in a pentapeptide is devised and validated. The method does not involve any prior knowledge of structural templates from sequence homology studies. Structural deformations can be predicted with about 70-80% reliability in any protein. Disease causing mutations and benign mutations have been addressed. In particular, p53, retinoblastoma protein and lipoprotein lipase are studied in detail. |
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Keywords: | SNPs Missense mutation Tripeptide Protein structure Disease Structural biology |
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