人胎脑源性神经前体细胞致瘤性评价

目的:研究体外培养的人胎脑源性神经前体细胞的致瘤性。方法:将人胎脑源神经前体细胞体外培养至第1、25、40、60代,且每代细胞分别制备成神经球及单个细胞混悬液两种制剂,并取293T细胞作为阳性对照,共9组,每组5只,分别皮下接种于4~8周龄的BALB/C裸鼠,接种后饲养6个月,定期观察裸鼠精神状态、饮食、排便、以及接种局部有无出现结节或肿块,接种6个月后处死裸鼠,对接种局部及内脏进行组织病理切片及HE染色。结果:将人胎脑源性神经前体细胞接种于裸鼠皮下6个月未见肿瘤形成,且未见其他异常组织形成;而阳性对照组293T细胞接种于裸鼠皮下1个月后可见明显肿瘤形成。结论:人胎脑源神经前体细胞对裸鼠不具有体内致瘤性。

Tumorigenicity of Human Fetal Brain-derived Neural Precursor Cells

Objective:To study the tumorigenicity of the human fetal brain-derived neural precursor cells cultured in vitro.Methods:The human fetal brain-derived neural precursor cells were cultured to passage 1, 25, 40 and 60 in vitro, and each passage of themwere prepared as two types of preparations of neurospheres and single cell suspension, and then 293T cells were taken as a positive control. BALB/C nude mice aged 4-8 weeks were assigned to 9 groups, and each group has 5 ones. They underwent subcutaneous inoculation respectively. The nude mice were fed for 6 months after inoculation, and their mental state, diet, defecation as well as the presence of nodules or lumps in the local inoculation parts were observed. They were killed six months after inoculation, then local inoculation parts and viscera were treated with histopathologic slicing and HE staining.Results:No tumor or other abnormal tissue formed 6 months after the subcutaneous injection of human fetal brain-derived neural precursor cells in nude mice. However, visible tumor was formed 1 month after the subcutaneous injection of 293T cells in nude mice in positive control group.Conclusion:The human fetal brain-derived neural precursor cells in vivo in nude mice tumorigenicity experiments indicated no tumorigenicity.