大鼠腹腔异位心脏移植模型建立及改进

目的:建立一种快速有效的大鼠腹腔异位心脏移植模型。方法:采用SD大鼠作为受体,Wistar大鼠作为供体,行同种异位腹腔心脏移植,术后给以CsA5 mg/kg/d灌服,心脏移植手术方法采用改良的Ono术式,观察改良的腹腔异位心脏移植各步骤所需时间、术后成功率及主要并发症发生率。结果:共建立40只大鼠异位心脏移植模型,手术成功率92.5%。动脉吻合时间12.5±2.3min,静脉吻合时间12.3±1.5 min,供心缺血时间37±3.5 min,受体血管阻断时间34.2±2.6 min,总手术时间90.2±4.8 min,出现的主要并发症为出血和供心复跳失败(各占5%、2.5%)。结论:改进的大鼠腹部异位心脏移植技术是一种简便、快速、有效、成功率高的模型制作方法。

The Establishment and Improvement of Heterotopic Heart TransplantationModel in Rats

Objective:To establish a rapid and effective heterotopic heart transplant model in rats.Methods:Allogeneicabdominal heterotopic heat transplantation was performed to make the SD rats as recipients and Wistar rats as the donors. After surgery,the rats were given the CsA5 mg/kg/d by gavage. The surgery of Ono style was further optimized based on the abdominal heterotopicheart transplantation, the time for each step, success rate and main complications after surgery.Results:40 rats'' heterotopic hearttransplantation models were established with the success rate of 92.5%, and the aortic anastomosis time was 12.5 ± 2.3 min, thepulmonary artery anastomosis time was 12.3± 1.5 min, total donor heart ischemia time was 37± 3.5 min, vessel occlusion time was34.2± 2.6 min, and total time surgery was 90.2± 4.8 min. The main complications were bleeding and/or resuscitation for heart failurewhich occurred to 5%and 2.5%of experimental rats, respectively.Conclusion:Improved abdominal heterotopic heart transplantation isa simple, rapid, effective and high success rate method of model establishment.