Improved protocols for functional analysis in the pathogenic fungus <Emphasis Type="Italic">Aspergillus flavus</Emphasis> |
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Authors: | Zhu-Mei He Michael S Price Gregory R OBrian D Ryan Georgianna Gary A Payne |
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Institution: | (1) Department of Plant Pathology, North Carolina State University, 27695 Raleigh, NC, USA;(2) School of Life Sciences, Sun Yat-Sen University, 510275 Guangzhou, P. R. China;(3) Department of Molecular Genetics and Microbiology, Duke University Medical Center, 27710 Durham, NC, USA |
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Abstract: | Background An available whole genome sequence for Aspergillus flavus provides the opportunity to characterize factors involved in pathogenicity and to elucidate the regulatory networks involved
in aflatoxin biosynthesis. Functional analysis of genes within the genome is greatly facilitated by the ability to disrupt
or mis-express target genes and then evaluate their result on the phenotype of the fungus. Large-scale functional analysis
requires an efficient genetic transformation system and the ability to readily select transformants with altered expression,
and usually requires generation of double (or multi) gene deletion strains or the use of prototrophic strains. However, dominant
selectable markers, an efficient transformation system and an efficient screening system for transformants in A. flavus are absent. |
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