Xylitol production by a Pichia stipitis
D-xylulokinase mutant |
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| Authors: | Yong-Su Jin Jose Cruz Thomas W Jeffries |
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| Institution: | (1) Department of Food Science, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, USA;(2) Department of Chemical Engineering, University of Vigo, As Lagoas, 32004 Ourense, Spain;(3) Present address: USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53726-2398, USA;(4) Present address: Present address: Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-422, 77 Massachusetts Ave, Cambridge, MA 02139, USA |
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| Abstract: | Xylitol production by Pichia stipitis FPL-YS30, a xyl3- 1 mutant that metabolizes xylose using an alternative metabolic pathway, was investigated under aerobic and oxygen-limited culture conditions. Under both culture conditions, FPL-YS30 (xyl3-1) produced a negligible amount of ethanol and converted xylose mainly into xylitol with comparable yields (0.30 and 0.27 g xylitol/g xylose). However, xylose consumption increased five-fold under aerobic compared to oxygen-limited conditions. This suggests that the efficiency of the alternative route of xylose assimilation is affected by respiration. As a result, the FPL-YS30 strain produced 26 g/l of xylitol, and exhibited a higher volumetric productivity (0.22 g xylitol l–1 h–1) under aerobic conditions. |
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