LRP16对乳腺癌MCF-7细胞增殖的影响

用Northern印迹方法检测雌二醇 (17β E2 )对LRP16mRNA表达的时间及剂量依赖性调控作用 .构建LRP16基因启动子序列调控的萤光素酶报告子 (pS0 ) ,并与雌激素受体α和 β(ERα和ERβ)表达载体共转染COS 7和MCF 7细胞后测定萤光素酶活性 .将LRP16基因的表达载体转染MCF 7细胞 ,测定过表达LRP16对细胞的生长特性的影响 .17β E2 使MCF 7细胞中LRP16mRNA表达水平增加 ,增加幅度未显示出 17β E2 培养时间和剂量的依赖性 .pS0 与ERα表达载体共转染细胞的相对萤光素酶活性较非共转染组 (对照组 )及pS0 ERβ表载体共转染组升高 5～ 10倍 .LRP16基因过表达促进MCF 7细胞的增殖 .研究表明 ,雌激素可能通过ERα上调乳腺癌MCF 7细胞LRP16基因的表达并促进细胞增殖

Effect of LRP16 on Breast Cancer MCF-7 Cell Proliferation

The expression level of LRP16 mRNA induced by 17β estrodiol (17β E 2) in MCF 7 cells was determined by Northern blot analysis. LRP16 promoter controlled luciferase expression vector (pS 0) was constructed and was co transfected with estrogen receptor α and β (ERα and ERβ) into COS 7 and MCF 7 cells, respectively, then the relative luciferase activity was measured. The effect of overexpression of LRP16 on MCF 7 cell proliferation was examined by trypan blue exclusion method in the cells transfected by LRP16. The results indicated that 17β E 2 increased the expression of LRP16 mRNA in MCF 7 cells, and this effect was not time and dose dependent. The relative luciferase activity in both COS 7 cells and MCF 7 cells co transfected by pGL3 S 0 and ERα was 5 to 10 fold of that in the control cells transfected by pS 0 alone or co transfected by pS 0 and ERβ. Overexpression of LRP16 promoted MCF 7 cell proliferation. These results suggested that estrogen up regulated the expression level of LRP16 mRNA through activating ERα and promoted MCF 7 cell proliferation.