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Cloning of chicken globin cDNA in bacterial plasmids |
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| Authors: | J Padayatty I Cummings C L Manske R Higuchi S Woo W Salser |
| Institution: | 1. Department of Biology and Molecular Biology Institute, University of California, Los Angeles, CA 90024, U.S.A.;2. Howard Hughes Medical Institute Laboratory, Baylor College of Medicine, Houston, TX 77030 U.S.A. |
| Abstract: | Chicken globin cDNA was prepared from mRNA isolated from phenylhydrazine-treated chicken reticulocytes, and cloned into the tetracycline-resistance gene of plasmid pMB9. Restriction enzyme analysis indicates that there are at least two classes of clones isolated. Nucleotide sequence data have shown that these classes corresponded to the chicken α- and β-globin genes. |
| Keywords: | Recombinant DNA restriction endonuclease mapping DNA sequencing hemoglobin mRNAs bp base pairs TE 0 01 M Tris ·HCl 0 001 M EDTA (pH 7 4) |
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