乳酸菌抑制葡聚糖硫酸钠诱导的结肠黏膜氧化应激损伤的研究

目的研究乳酸菌抗氧化活性与缓解葡聚糖硫酸钠(DSS)诱导结肠炎的关系。方法评价5株乳酸菌体外抑制大鼠肝细胞脂质过氧化活性,用DSS诱导小鼠结肠炎模型研究抗氧化性不同的菌株体内抗氧化活性;小鼠分成7组,分别是Control组、DSS组、L.plantarum Fn008+DSS组、L.casei Fn012+DSS组、L.acidophilus Fn022+DSS组、L.sakei Fn034+DSS组和L.acidophilus Fn037+DSS组。结果体外实验表明,Fn022和Fn034的丙二醛(MDA)抑制率高于LGG,但差异无统计学意义,Fn008和Fn037的MDA抑制率显著低于LGG,Fn012的MDA抑制率最低。体内实验表明,DSS可导致小鼠氧化应激,升高结肠内容物的自由基水平,降低结肠抗氧化能力;Fn008和Fn037预处理能显著降低DSS诱导结肠内容物的自由基水平,Fn008能显著降低结肠组织的髓过氧化物酶(MPO)水平,提高结肠抗氧化能力,Fn022和Fn034的干预能力居中,Fn012的干预能力最弱。相关性分析表明乳酸菌体外MDA清除率与体内MPO有负相关趋势(r=-0.862,P=0.06)。结论乳酸菌抗氧化活性与其在肠道抑制炎性细胞激活的作用有关,本实验中Fn008和Fn037具有显著的体内抗氧化作用。

Lactic acid bacteria inhibit mucosal oxidative stress of colon in dextran sulfate sodium-induced colitic mice

Objective To explore the relationship between in vitro antioxidant ability and anti-inflammatory effect in vivo in lactobacillus strain.Method Inhibition of lipid peroxide formation was studied in Fe2＋/ascorbate system in vitro.In vivo study was carried out in 7 groups of mice： control,DSS treatment,L.plantarum Fn008＋DSS treatment,L.casei Fn012＋DSS treatment,L.acidophilus Fn022＋DSS treatment,L.sakei Fn034＋DSS treatment and L.acidophilus Fn037 ＋DSS treatment.Result In vitro antioxidant abilities of Fn022 and Fn034 were better than that of LGG,while those of Fn008 and Fn037 were on sub-significance level less than LGG.DSS treatment significantly induced free radicals（ROS） production and decreased total antioxidant activity in colon lumen,and increased MPO production in colon mucosa.Fn008 and Fn037 inhibited lumen ROS increase.Fn008 significantly prevented mucosa MPO increasing.In vitro antioxidant ability of lactobacillus was negatively correlated with in vivo MPO inhibition（r=-0.862,P=0.06）.Conclusion Antioxidant ability has critical for anti-inflammation of lactobacillus in vivo.Fn008 and Fn037 show potential probiotic effect in this study.