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一株降血压功能乳酸菌在模拟胃肠环境中抗性的研究
引用本文:徐丽丹,邹积宏,袁杰利. 一株降血压功能乳酸菌在模拟胃肠环境中抗性的研究[J]. 中国微生态学杂志, 2011, 23(2): 112-114
作者姓名:徐丽丹  邹积宏  袁杰利
作者单位:1. 大连医科大学,微生态学教研室,辽宁,大连,116044;黑龙江大学生命科学学院,黑龙江,哈尔滨,150080
2. 黑龙江大学生命科学学院,黑龙江,哈尔滨,150080
3. 大连医科大学,微生态学教研室,辽宁,大连,116044
基金项目:国家"863"计划,国家"973"计划
摘    要:目的研究具有降血压功能乳酸菌DM9057株对pH、胆汁盐、胃蛋白酶及胰蛋白酶的耐受性。方法以MRS为基础培养基,模拟胃肠环境。结果 DM9057在pH2~3、胆汁盐浓度0.1%~0.3%、胃蛋白酶0.592~1.482μg/mL和胰蛋白酶14.24~72.32 U/g的条件下,37℃培养4 h,活菌数均能达到107CFU/mL以上。结论乳酸菌DM9057能顺利通过胃肠道,达到促进健康的作用。

关 键 词:乳酸菌DM9057  胃蛋白酶  胰蛋白酶  胆盐  抗性

The antagonistic properties of an antihypertensive Lactic acid bacteria in imitative gastroenteric environment
XU Li-dan,ZOU Ji-hong,YUAN Jie-li. The antagonistic properties of an antihypertensive Lactic acid bacteria in imitative gastroenteric environment[J]. Chinese Journal of Microecology, 2011, 23(2): 112-114
Authors:XU Li-dan  ZOU Ji-hong  YUAN Jie-li
Affiliation:1(1.Microecology Department of Dalian Medical University,Dalian 116044,China;2.College of Life Science,Heilongjiang University,Harbin 150080,China)
Abstract:Objective To study the tolerance of Lactobacillus DM9057 against pepsin,trypsin,bile salt and pH.Method MRS medium was used.Result The Lactobacillus DM9057 grow well in an environment as follows: pH was 2.0 to 3.0,the concentration of bile salt was 0.1% to 0.3%,the concentration of pepsin was 0.592 to 1.482 μg/mL,the concentration of trypsin was 14.24 to 73.32 U/g,temperature was at 37 ℃.After 4 hrs of cultivation the living bacterial count reached above 107 CFU/mL.Conclusion Lactobacillus DM9057 can go through the gastrointestinal environment,and help to improve people′s heath.
Keywords:Lactobacillus DM9057  Pepsin  Trypsin  Bile salt  Antagonistic property
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