结直肠癌患者肠道黏膜菌群多样性变化研究

目的应用PCR-DGGE技术对结直肠癌患者肠道黏膜局部菌群多样性进行研究,为结直肠病变的防治提供微生态调节思路。方法收集正常对照组、结直肠息肉组及结直肠癌组患者各30例,采集结直肠黏膜局部肛拭子,提取细菌基因组DNA,采用PCR-DGGE对肠道黏膜局部菌群进行指纹图谱分析。结果正常对照组、结直肠息肉组和结直肠癌组患者PCR-DGGE指纹图谱分析显示3组肠道黏膜局部菌群多样性发生了显著的变化,3组肠道黏膜局部菌群发生了显著的菌群变迁。结论 PCR-DGGE分析结直肠癌患者肠道黏膜局部菌群多样性变化对监测肠道局部微生态变化在结直肠癌的发生过程中的作用具有重要价值。

Diversity of intestinal bacterial microbiota in patients with colorectal cancer

Objective To explore the intestinal bacterial diversity in patients with colorectal cancer by PCR-DGGE technique,which might provide new micro-ecological insights for the prevention and treatment of colorectal lesions.Method Three groups including the control group,colorectal polyps group and colorectal cancer group were enrolled in the study.After examination,the colorectal local anal swabs were collected,bacterial genomic DNA was extracted,and the diversity of intestinal microbiota was analyzed with PCR-DGGE.Result With PCR-DGGE fingerprinting analysis,the diversity of intestinal microbiota was significantly different among the three groups and showed a slight of shift of microbiota.Conclusion The diversity of intestinal microbiota from colorectal cancer patients with PCR-DGGE plays an important role in monitoring the development of colorectal lesions.