Two pathways of plant regeneration in wheat anther culture |
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| Authors: | Konieczny R Czaplicki AZ Golczyk H Przywara L |
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| Institution: | (1) Department of Plant Cytology and Embryology, Jagiellonian University, ul. Grodzka 52, 31-044 Kraków, Poland;(2) Plant Breeding and Acclimatization Institute, 05-870 Blonie, Poland |
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| Abstract: | The anthers of 10 Polish winter wheat (Triticum aestivum L.) cultivars were used for the induction of androgenesis and plant regeneration. The highest rate of callus induction (9.1%) and green plant production (0.8%) was obtained with the cultivar Apollo that was chosen for histological analysis. The first androgenic division was symmetrical and occurred after 3 weeks of culture. Further divisions of newly formed cells gave rise to multicellular structures which followed two developmental pathways: callus production or direct embryo formation. Plant regeneration was observed in both pathways. Chromosome counting of plantlets regenerated showed that haploid metaphases 2n=3x=21 were the most frequent. |
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| Keywords: | Androgenesis Anther culture Gramineae Organogenesis Plant regeneration Triticum aestivum |
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