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| H6亚型禽流感病毒RT-LAMP检测方法的建立 | |
| 引用本文： | 周辰瑜,谢芝勋,宋杨,彭宜,陈安莉,刘加波,庞耀珊,邓显文,谢志勤,谢丽基. H6亚型禽流感病毒RT-LAMP检测方法的建立[J]. 微生物学通报, 2011, 38(12): 1855-1861 |
| 作者姓名： | 周辰瑜 谢芝勋 宋杨 彭宜 陈安莉 刘加波 庞耀珊 邓显文 谢志勤 谢丽基 |
| 作者单位： | 1. 广西大学动物科学技术学院广西南宁 530004 2. 广两兽医研究所 广西南宁530001 3. 广西南宁市动物卫生监督所 广西南宁530001 |
| 基金项目： | 国家百千万人才工程人选专项资金项目(No. 945200603); 广西科技专项资金项目(No. 桂科专项10-3) |
| 摘 要： | 建立一种快速、敏感、特异的检测H6亚型禽流感病毒(AIV)逆转录环介导等温扩增(RT-LAMP)检测方法.根据H6亚型AIV血凝素(HA)基因序列的保守区8个位点设计了6条特异性LAMP引物,以H6亚型AIV阳性样品RNA为模板进行一步法扩增,对反应条件和反应体系进行优化.结果表明该方法的特异性良好,对其他呼吸道病原体均无扩增反应.该方法灵敏度高,最低可检测到H6亚型AIV RNA为0.01 pg,该方法无需特殊仪器,只需在水浴锅中进行,是一种适于基层的简便、灵敏、快速的H6亚型AIV检测方法. |
| 关 键 词： | H6亚型禽流感病毒 逆转录环介导等温扩增 特异性 敏感性 |
Establishment of a reverse transcription loop-mediated isothermal amplification assay for detection of H6 subtype avian influenza virus |
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| ZHOU Chen-Yu,XIE Zhi-Xun,SONG Yang,PENG Yi,CHEN An-Li,LIU Jia-Bo,PANG Yao-Shan,DENG Xian-Wen,XIE Zhi-Qin and XIE Li-Ji. Establishment of a reverse transcription loop-mediated isothermal amplification assay for detection of H6 subtype avian influenza virus[J]. Microbiology China, 2011, 38(12): 1855-1861 | |
| Authors: | ZHOU Chen-Yu XIE Zhi-Xun SONG Yang PENG Yi CHEN An-Li LIU Jia-Bo PANG Yao-Shan DENG Xian-Wen XIE Zhi-Qin XIE Li-Ji |
| Affiliation: | 1. College of Veterinary Medicine, Guangxi University, Nanning, Guangxi 530004, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;3. GuangXi Animal Health Inspection, Nanning, Guangxi 530001, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;1. College of Veterinary Medicine, Guangxi University, Nanning, Guangxi 530004, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China;2. GuangXi Veterinary Research Institute, Nanning, Guangxi 530001, China |
| Abstract: | |
| Keywords: | H6 subtype avian influenza virus RT-LAMP Specific Sensitive |
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