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Detection of head-to-tail DNA sequences of human bocavirus in clinical samples
Authors:Lüsebrink Jessica  Schildgen Verena  Tillmann Ramona Liza  Wittleben Felix  Böhmer Anne  Müller Andreas  Schildgen Oliver
Institution:1. Institut für Pathologie, Kliniken der Stadt Köln gGmbH, Klinikum der Privaten Universität Witten-Herdecke, Köln (Cologne), Germany.; 2. Department of Paediatrics, University Hospital Bonn, Bonn, Germany.;Kantonal Hospital St. Gallen, Switzerland
Abstract:Parvoviruses are single stranded DNA viruses that replicate in a so called "rolling-hairpin" mechanism, a variant of the rolling circle replication known for bacteriophages like φX174. The replication intermediates of parvoviruses thus are concatemers of head-to-head or tail-to-tail structure. Surprisingly, in case of the novel human bocavirus, neither head-to-head nor tail-to-tail DNA sequences were detected in clinical isolates; in contrast head-to-tail DNA sequences were identified by PCR and sequencing. Thereby, the head-to-tail sequences were linked by a novel sequence of 54 bp of which 20 bp also occur as conserved structures of the palindromic ends of parvovirus MVC which in turn is a close relative to human bocavirus.
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