Agrobacterium-mediated transient transformation of marigold (Tagetes erecta) |
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Authors: | Gregorio Godoy-Hernández Elide Avilés Berzunza Lizbeth Castro Concha María de Lourdes Miranda-Ham |
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Affiliation: | (1) Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Chuburná de Hidalgo, Mérida, Yucatán, 97200, México |
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Abstract: | Hypocotyls, roots, leaf sections and shoot tips from Tagetes erecta plantlets were inoculated with Agrobacterium tumefaciens, harboring the binary vector pCAMBIA2301, containing the β-glucuronidase gene. Histochemical GUS assays of infected tissues showed transient gus gene expression after 3 days. |
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Keywords: | β -glucuronidase genetic transformation marigold neomycin phosphotransferase II Tagetes erecta |
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