In vitro insertion of the myelin proteolipid apoprotein into oligodendrocyte plasma membranes |
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Authors: | Zakaria Chraibi Annette Alfsen Françoise Lavialle |
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Affiliation: | (1) UPR 64 Cnrs, UFR Biomédicale des Saints-Pères, 45, rue des Saints Pères, 75270 Paris Cedex 06, France |
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Abstract: | Uncoated vesicles (UCV) loaded with the myelin proteolipid apoprotein covalently tagged with fluorescein (PLPF) were found to interact with isolated oligodendrocytes from bovine brain at 4°C as well as at 37°C. After 1.5 hours of incubation, the labeled protein was localized in the cell membranes. After 2.5 hours the fluorescence intensity associated with the oligodendrocytes decreased and completely disappeared at t=3.5 hours. Addition of KCl or EDTA in the incubation medium significantly hindered the interaction with cells. In contrast, the elimination of membrane proteins from UCV did not perturb cell labeling. A specific role of PLP was suggested since UCV loaded with a soluble protein (BSAF) led to a weak cell labeling.Abbreviations IAF 5-iodacetamidofluorescein - BSA bovine serum albumin - BSA BSA labelled with IAF - PLP proteolipid apoprotein - PLPF aqueous form of PLP tagged with IAF - CV coated vesicles - UCV uncoated vesicles - UCV*PLPF UCV loaded with PLPF - MV model vesiclesThis work was suported by Cnrs and INSERM. |
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Keywords: | Fusion vesicles oligodendrocytes myelin proteolipid |
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