Immunohistochemical demonstration of airway epithelial cell markers of guinea pig |
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Authors: | Li Yong Wang Jing He Hai Yan Ma Ling Jie Zeng Jin Deng Guang Cun Liu Xiaoming Engelhardt John F Wang Yujiong |
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Institution: | aKey Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, China;bCollege of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China;cGraduate School, Ningxia University, Yinchuan, Ningxia 750021, China;dDepartment of Pathology, Ningxia People's Hospital, Yinchuan, Ningxia 750021, China;eDepartment of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242, USA |
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Abstract: | The guinea pig (Cavea porcellus) is a mammalian non-rodent species in the Caviidae family. The sensitivity of the respiratory system and the susceptibility to infectious diseases allows the guinea pig to be a useful model for both infectious and non-infectious lung diseases such as asthma and tuberculosis. In this report, we demonstrated for the first time, the major cell types and composition in the guinea pig airway epithelium, using cell type-specific markers by immunohistochemical staining using the commercial available immunological reagents that cross-react with guinea pig. Our results revealed the availability of antibodies cross-reacting with airway epithelial cell types of basal, non-ciliated columnar, ciliated, Clara, goblet and alveolar type II cells, as well as those cells expressing Mucin 5AC, Mucin 2, Aquaporin 4 and Calcitonin Gene Related Peptide. The distribution of these various cell types were quantified in the guinea pig airway by immunohistochemical staining and were comparable with morphometric studies using an electron microscopy assay. Moreover, this study also demonstrated that goblet cells are the main secretory cell type in the guinea pig's airway, distinguishing this species from rats and mice. These results provide useful information for the understanding of airway epithelial cell biology and mechanisms of epithelial–immune integration in guinea pig models. |
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Keywords: | Guinea pig Epithelial cells Airway Lung Immunohistochemical staining Morphometry |
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