Endocytosis of micro- and nanosized particles in vitro by human dendritic cells |
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Authors: | P A Karalkin A Yu Lupatov K N Yarygin |
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Institution: | 1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10, Moscow, 119121, Russia 2. Russian State Medical University, ul. Ostrovityanova 1, Moscow, 117997, Russia
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Abstract: | The ability of human dendritic cells (DC) to uptake synthetic micro- and nanosized particles was assessed by flow cytometry
and fluorescent microscopy. DCs were differentiated in vitro from blood monocytes in the presence of recombinant cytokines.
Further maturation of DC in culture after the addition of maturation factors resulted in the increased expression of HLA-DR
and co-stimulatory molecules CD80, CD83, CD86, in comparison with immature DC. Active internalization of Fluoresbrite-YG fluorescent
microbeads (0.2 μm) was noted for immature but not mature DCs. The decrease of endocytic activity after DC maturation correlated
with the reduced expression of CD209, the surface membrane receptor participating in phagocytosis. Unlike microparticles,
the uptake of nanoscale Quantum dots-655 did not depend on the stage of DC maturation and probably was mediated by a different
endocytosis mechanism. |
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