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Mechanism of S-(carboxymethylthio)cysteine cleavage by rat liver cystathionine-gamma-lyase. |
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| Authors: | K Yao M Kinuta Y Watanabe |
| Abstract: | Rat liver cystathionine-gamma-lyase [L-cystathionine cysteinelyase (deaminating), EC 4.4.1.1] catalyzes the formation of pyruvic acid, ammonia, and carboxymethylhydrodisulfide from S-(carboxymethylthio)cysteine (CMTC). As judged by pyruvic acid production, the optimal pH is 8.3 in tris-HCl buffer and the Km is 2.9 mM. A possible mechanism of CMTC cleavage by cystathionase is proposed. |
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