Production of 2-hydroxy 4-methoxy benzaldehyde using root cultures of Hemidesmus indicus |
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Authors: | S. Sreekumar S. Seeni P. Pushpangadan |
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Affiliation: | (1) Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Pacha-PalodeThiruvananthapuram, India |
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Abstract: | Nodal explants of Hemidesmus indicus plants cultured in half strength Murashige and Skoog medium fortified with 2 mg indole-3-butyric acid l–1 in the dark produced 10–12 roots (1–2 cm) with minimal callusing in 10 days. Roots, 20 mg dry wt, cultured in Gamborg et al. medium supplemented with 2 mg IBA l–1, sucrose (4% w/v), pH (5.6) and agitation (70 rpm) in the dark yielded 550 mg root dry wt/flask and 0.18% 2-hydroxy 4-methoxy benzaldehyde in the roots after 30 days. The maintenance of normal morphology of the roots, consistent biomass and product yields over a 21-month period indicated the stability of the cultures. © Rapid Science Ltd. 1998 |
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